DNA Extraction From Chicken Liver Assignment

DNA Extraction From Chicken Liver: Deoxyribonucleic acid (DNA) is the hereditary material in humans and almost all other organisms. Nearly every cell in a person’s body has the same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitochondria (where it is called mitochondrial DNA or mtDNA).

DNA Extraction From Chicken Liver NB: This essay is a result of a test that we gave our recruits. If we want our professional writers to handle your assignment, kindly ORDER NOW below.

The information in DNA is stored as a code made up of four chemical bases: adenine (A), guanine (G), cytosine (C), and thymine (T). Human DNA consists of about 3 billion bases, and more than 99 percent of those bases are the same in all people. The order, or sequence, of these bases determines the information available for building and maintaining an organism, similar to the way in which letters of the alphabet appear in a certain order to form words and sentences.

DNA bases pair up with each other, A with T and C with G, to form units called base pairs. Each base is also attached to a sugar molecule and a phosphate molecule. Together, a base, sugar, and phosphate are called a nucleotide. Nucleotides are arranged in two long strands that form a spiral called a double helix. The structure of the double helix is somewhat like a ladder, with the base pairs forming the ladder’s rungs and the sugar and phosphate molecules forming the vertical sidepieces of the ladder.

An important property of DNA is that it can replicate, or make copies of itself. Each strand of DNA in the double helix can serve as a pattern for duplicating the sequence of bases. This is critical when cells divide because each new cell needs to have an exact copy of the DNA present in the old cell.

The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA that allow scientists to detect genetic disorders, produce DNA fingerprints of individuals, and even create genetically engineered organisms that can produce beneficial products such as insulin, antibiotics, and hormones.Â

Once the DNA has been isolated, it is essential to accurately determine its concentration for subsequent manipulation such as cloning or sequence determination.

To quantify the amount of DNA that extracted by using spectrophotometry.

The aims of this experience is to:

  • To use the properties of DNA to isolate long strands of DNA from liver cells.
  • To determine the yield of DNA isolated from a given amount of tissue.
  • To examine the light absorbing properties of purified DNA.
  • To examne the relationship between the concentration of a DNA solution and the absorbnce at 595nm of DNA-diphenylamine solution.
  • To generate a standrad curve relating DNA concentraton with the absorbance of DNA-diphenylamine solutions.
  • To use a standard curve to determine the concentration of an unknown DNA solution.

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